Journal: Journal of Medicinal Chemistry

Article Title: Copper(II) Complexes with Isomeric Morpholine-Substituted 2-Formylpyridine Thiosemicarbazone Hybrids as Potential Anticancer Drugs Inhibiting Both Ribonucleotide Reductase and Tubulin Polymerization: The Morpholine Position Matters

doi: 10.1021/acs.jmedchem.4c00259

Figure Lengend Snippet: (a) UV–vis spectra for the copper(II)– H 2 L 4 (1:1) system with an increasing concentration of EDTA. (b) Measured (filled circle) and fitted (dashed line) absorbances at 420 nm plotted against the EDTA-to-ligand ratio ( c L = 25 μM, c Cu = 25 μM, c EDTA = 0–62.5 μM, 30% (v/v) DMSO/H 2 O; pH = 5.90, I = 0.1 M (KCl), t = 25 °C).

Article Snippet: The cell was positioned in the CUV-UV Cuvette Holder (Ocean Optics) connected to the diode array UV–vis-NIR spectrometer by optical fibers.

Techniques: Concentration Assay

Journal: Journal of Medicinal Chemistry

Article Title: Copper(II) Complexes with Isomeric Morpholine-Substituted 2-Formylpyridine Thiosemicarbazone Hybrids as Potential Anticancer Drugs Inhibiting Both Ribonucleotide Reductase and Tubulin Polymerization: The Morpholine Position Matters

doi: 10.1021/acs.jmedchem.4c00259

Figure Lengend Snippet: (a) Time-dependent UV–vis absorption spectra of complex 6 (copper(II)– H 2 L 6 (1:1) system) in the presence of GSH (50 equiv) before (red line) and after mixing the solutions (black/gray lines) in a tandem cuvette under anoxic conditions. The absorbance changes after bubbling oxygen into the reaction mixture (blue line). (b) Plot of the time-dependent absorbance changes at 406 nm for 6 (cross, black line), at 396 nm for 4 (square, green line), at 398 nm for 5 (circle, red line), and at 398 nm for 3 (triangle, blue line) (50 mM HEPES, pH = 7.4); solvent: water; c Cu = c L = 25 μM; c GSH = 1.25 mM; I = 0.1 M (KCl); t = 25 °C).

Article Snippet: The cell was positioned in the CUV-UV Cuvette Holder (Ocean Optics) connected to the diode array UV–vis-NIR spectrometer by optical fibers.

Techniques: Solvent

Journal: Journal of Medicinal Chemistry

Article Title: Copper(II) Complexes with Isomeric Morpholine-Substituted 2-Formylpyridine Thiosemicarbazone Hybrids as Potential Anticancer Drugs Inhibiting Both Ribonucleotide Reductase and Tubulin Polymerization: The Morpholine Position Matters

doi: 10.1021/acs.jmedchem.4c00259

Figure Lengend Snippet: (a) CVs of 6 (black trace—first scan, red trace—second scan) in DMSO/ n -Bu 4 NPF 6 at the Pt working electrode at a scan rate of 100 mV s –1 . (b) UV–vis spectra measured upon cathodic reduction of 6 at the first reduction peak by using a honeycomb Pt working electrode (inset: (black trace) EPR spectrum of 6 in DMSO; (red and blue trace) EPR spectra of 6 in DMSO after cathodic reduction at the first cathodic peak.)

Article Snippet: The cell was positioned in the CUV-UV Cuvette Holder (Ocean Optics) connected to the diode array UV–vis-NIR spectrometer by optical fibers.

Techniques: